Overview

  • Product name

  • Description

    Goat polyclonal to PCBP4
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human PCBP4 (C terminal). NP_065151.1; NP_127501.1; NP_127502.1; NP_127503.1.
    Sequence:

    CNGSKKAERQKFSPY

  • Positive control

    • WB: HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab63948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 44 kDa).
ELISA 1/128000.

Target

Images

  • Anti-PCBP4 antibody (ab63948) at 1 µg/ml (1 hour) + HeLa cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 41 kDa
    why is the actual band size different from the predicted?

References

ab63948 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Negative control
Protein G dynabeads without antibody bound.
Immuno-precipitation step
Other - Protein G dynabeads
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
hnRNP C antibody (sc-15386) bound protein G dynabeads 0.1 mg/ml (antibody/beads).

Abcam user community

Verified customer

Submitted Aug 18 2011

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