For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    pcca-antibody-c-terminal-ab187686.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Amino Acids
Share by email

Anti-PCCA antibody - C-terminal (ab187686)

  • Datasheet
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-PCCA antibody - C-terminal (ab187686)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)

Key features and details

  • Rabbit polyclonal to PCCA - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human PCCA protein (ab159059)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PCCA antibody - C-terminal
    See all PCCA primary antibodies
  • Description

    Rabbit polyclonal to PCCA - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human PCCA aa 678-728 (C terminal). The exact sequence is proprietary.
    Sequence:

    GDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVEL E


    Database link: P05165
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates. IHC-P: Human lung cancer and ovarian carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab187686 was affinity purified using an epitope specific to PCCA immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human PCCA protein (ab159059)
  • Related Products

    • Recombinant Human PCCA protein (ab159059)

Applications

Our Abpromise guarantee covers the use of ab187686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Pathway

    Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.
  • Involvement in disease

    Defects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein.
  • Sequence similarities

    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P05165 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 418774 Chicken
    • Entrez Gene: 738775 Chimpanzee
    • Entrez Gene: 614302 Cow
    • Entrez Gene: 476975 Dog
    • Entrez Gene: 101127469 Gorilla
    • Entrez Gene: 100062251 Horse
    • Entrez Gene: 5095 Human
    • Entrez Gene: 110821 Mouse
    • Entrez Gene: 100456529 Orangutan
    • Entrez Gene: 100524103 Pig
    • Entrez Gene: 687008 Rat
    • Entrez Gene: 699844 Rhesus monkey
    • Entrez Gene: 437019 Zebrafish
    • Omim: 232000 Human
    • SwissProt: P05165 Human
    • SwissProt: Q91ZA3 Mouse
    • SwissProt: P14882 Rat
    • Unigene: 80741 Human
    • Unigene: 23876 Mouse
    • Unigene: 6033 Rat
    see all
  • Alternative names

    • mitochondrial antibody
    • Pcca antibody
    • pccA COMPLEMENTATION GROUP antibody
    • PCCA_HUMAN antibody
    • PCCase subunit alpha antibody
    • Propanoyl CoA:carbon dioxide ligase subunit alpha antibody
    • Propanoyl-CoA:carbon dioxide ligase subunit alpha antibody
    • Propionyl CoA carboxylase alpha chain antibody
    • Propionyl CoA carboxylase alpha chain mitochondrial antibody
    • Propionyl CoA carboxylase alpha polypeptide antibody
    • Propionyl coenzyme A carboxylase alpha polypeptide antibody
    • Propionyl-CoA carboxylase alpha chain antibody
    see all

Images

  • Western blot - Anti-PCCA antibody - C-terminal (ab187686)
    Western blot - Anti-PCCA antibody - C-terminal (ab187686)
    All lanes : Anti-PCCA antibody - C-terminal (ab187686) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 80 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)

    Immunohistochemistry of human lung cancer tissue staining PCCA with an187686 at 1:1000. Detected using DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)

    Immunohistochemistry of human ovarian carcinoma tissue staining PCCA with an187686 at 1:1000. Detected using DAB.

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab187686? Please let us know so that we can cite the reference in this datasheet.

    ab187686 has been referenced in 1 publication.

    • Lim KH  et al. Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expression. Nat Commun 11:3501 (2020). PubMed: 32647108

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab187686.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.