Anti-PCCA antibody - C-terminal (ab187686)
Key features and details
- Rabbit polyclonal to PCCA - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PCCA antibody - C-terminal
See all PCCA primary antibodies -
Description
Rabbit polyclonal to PCCA - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human PCCA aa 678-728 (C terminal). The exact sequence is proprietary.
Sequence:GDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVEL E
Database link: P05165 -
Positive control
- WB: HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates. IHC-P: Human lung cancer and ovarian carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab187686 was affinity purified using an epitope specific to PCCA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab187686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 80 kDa. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Pathway
Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3. -
Involvement in disease
Defects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein. -
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 418774 Chicken
- Entrez Gene: 738775 Chimpanzee
- Entrez Gene: 614302 Cow
- Entrez Gene: 476975 Dog
- Entrez Gene: 101127469 Gorilla
- Entrez Gene: 100062251 Horse
- Entrez Gene: 5095 Human
- Entrez Gene: 110821 Mouse
see all -
Alternative names
- mitochondrial antibody
- Pcca antibody
- pccA COMPLEMENTATION GROUP antibody
see all
Images
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Western blot - Anti-PCCA antibody - C-terminal (ab187686)All lanes : Anti-PCCA antibody - C-terminal (ab187686) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)Immunohistochemistry of human lung cancer tissue staining PCCA with an187686 at 1:1000. Detected using DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)Immunohistochemistry of human ovarian carcinoma tissue staining PCCA with an187686 at 1:1000. Detected using DAB.
Datasheets and documents
References (1)
ab187686 has been referenced in 1 publication.
- Lim KH et al. Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expression. Nat Commun 11:3501 (2020). PubMed: 32647108
