Anti-PCCA antibody - C-terminal (ab187686)
Key features and details
- Rabbit polyclonal to PCCA - C-terminal
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PCCA antibody - C-terminal
See all PCCA primary antibodies -
Description
Rabbit polyclonal to PCCA - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human PCCA aa 650 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P05165 -
Positive control
- WB: HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates. IHC-P: Human lung cancer and ovarian carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab187686 was affinity purified using an epitope specific to PCCA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/500 - 1/2000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
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| Notes |
|---|
|
IHC-Fr
1/500 - 1/2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 80 kDa. |
Target
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Pathway
Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3. -
Involvement in disease
Defects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein. -
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 418774 Chicken
- Entrez Gene: 738775 Chimpanzee
- Entrez Gene: 614302 Cow
- Entrez Gene: 476975 Dog
- Entrez Gene: 101127469 Gorilla
- Entrez Gene: 100062251 Horse
- Entrez Gene: 5095 Human
- Entrez Gene: 110821 Mouse
see all -
Alternative names
- mitochondrial antibody
- Pcca antibody
- pccA COMPLEMENTATION GROUP antibody
see all
Images
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Western blot - Anti-PCCA antibody - C-terminal (ab187686)All lanes : Anti-PCCA antibody - C-terminal (ab187686) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Frozen sections) - Anti-PCCA antibody - C-terminal (ab187686)Immunohistochemistry of human lung cancer tissue staining PCCA with an187686 at 1:1000. Detected using DAB.
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Immunohistochemistry (Frozen sections) - Anti-PCCA antibody - C-terminal (ab187686)Immunohistochemistry of human ovarian carcinoma tissue staining PCCA with an187686 at 1:1000. Detected using DAB.
Datasheets and documents
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Datasheet download
References (1)
ab187686 has been referenced in 1 publication.
- Lim KH et al. Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expression. Nat Commun 11:3501 (2020). PubMed: 32647108
