Anti-PCCA antibody - C-terminal (ab187686)

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Overview

  • Product name

    Anti-PCCA antibody - C-terminal
    See all PCCA primary antibodies

  • Description

    Rabbit polyclonal to PCCA - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide within Human PCCA aa 678-728 (C terminal). The exact sequence is proprietary.
    Sequence:

    GDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVEL E


    Database link: P05165

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates. IHC-P: Human lung cancer and ovarian carcinoma tissue

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab187686 was affinity purified using an epitope specific to PCCA immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-PCCA antibody - C-terminal (ab187686)
      Western blot - Anti-PCCA antibody - C-terminal (ab187686)
      All lanes : Anti-PCCA antibody - C-terminal (ab187686) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate
      Lane 3 : Jurkat whole cell lysate
      Lane 4 : TCMK1 whole cell lysate
      Lane 5 : NIH 3T3 whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 80 kDa


      Exposure time: 3 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)

      Immunohistochemistry of human lung cancer tissue staining PCCA with an187686 at 1:1000. Detected using DAB.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody - C-terminal (ab187686)

      Immunohistochemistry of human ovarian carcinoma tissue staining PCCA with an187686 at 1:1000. Detected using DAB.

    References

    ab187686 has not yet been referenced specifically in any publications.

    Publishing research using ab187686? Please let us know so that we can cite the reference in this datasheet.

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