Key features and details
- Mouse polyclonal to PCCB
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PCCB antibody
See all PCCB primary antibodies
DescriptionMouse polyclonal to PCCB
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Full length human PCCB protein (NP_000523.2).
- PCCB transfected 293T lysate and human liver tissue lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab70416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
PathwayMetabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.
Involvement in diseaseDefects in PCCB are the cause of propionic acidemia type II (PA-2) [MIM:606054]. PA-2 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein.
Sequence similaritiesBelongs to the AccD/PCCB family.
Contains 1 carboxyltransferase domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- DKFZp451E113 antibody
- PCCase subunit beta antibody
- pccB antibody
Anti-PCCB antibody (ab70416) at 1/500 dilution + human liver tissue lysate at 25 µg
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
All lanes : Anti-PCCB antibody (ab70416) at 1/500 dilution
Lane 1 : PCCB transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 58 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
IHC image of ab70416 staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab70416 has not yet been referenced specifically in any publications.