Product nameAnti-PCDH15 antibody [EPR19600]
See all PCDH15 primary antibodies
DescriptionRabbit monoclonal [EPR19600] to PCDH15
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse PCDH15 aa 1-300. The exact sequence is proprietary.
Database link: Q99PJ1
- WB: 293T whole cell lysate transfected with a DDDK-tagged mouse PCDH15 construct . ICC/IF: HEK-293T cells transfected with a GFP-tagged mouse PCDH15 expression construct.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab202560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 260 kDa (predicted molecular weight: 215 kDa).|
Cellular localizationCell Membrane
- Cadherin related family member 15 antibody
- CDHR15 antibody
- DFNB23 antibody
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a GFP-tagged mouse PCDH15 expression construct or GFP vector only, labeling PCDH15 with ab202560 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
Confocal image showing positive staining on HEK-293 cells transfected with a GFP-tagged mouse PCDH15 expression construct.
This mouse PCDH15 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 594) (ab150080) at 1/1000 dilution.
All lanes : Anti-PCDH15 antibody [EPR19600] (ab202560) at 1/1000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with an empty expression vector
Lane 2 : 293T whole cell lysate transfected with a DDDK-tagged mouse PCDH15 construct
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 215 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 16481439).
The mouse PCDH15 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.
WB detection of endogenous PCDH15 is not shown due to unavailability of appropriate testing material.
ab202560 has not yet been referenced specifically in any publications.