Anti-PCDH17 antibody (ab128815)

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Western blot - Anti-PCDH17 antibody (ab128815)

    Key features and details

    • Goat polyclonal to PCDH17
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Overview

    • Product name

      Anti-PCDH17 antibody

    • Description

      Goat polyclonal to PCDH17

    • Host species

      Goat

    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data

    • Immunogen

      Synthetic peptide:

      C-RVFADVHSRASRDS

      , corresponding to C terminal amino acids 1098-1111 of Human PCDH17 (NP_001035519.1).
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Mouse fetal brain lysate

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 99% Tris buffered saline, 0.5% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab128815 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    All applications
    Rat
    Cow
    Human
    Pig
    Chinese hamster
    Application Abreviews Notes
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).

    1 hour primary incubation is recommended for this product.
    Notes
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).

    1 hour primary incubation is recommended for this product.

    Target

    Images

    • Western blot - Anti-PCDH17 antibody (ab128815)
      Western blot - Anti-PCDH17 antibody (ab128815)
      Anti-PCDH17 antibody (ab128815) at 0.2 µg/ml + Mouse fetal brain lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 126 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?


      Exposure time: 1 hour

    References (2)

    Publishing research using ab128815? Please let us know so that we can cite the reference in this datasheet.

    ab128815 has been referenced in 2 publications.

    • Liu S  et al. PCDH17 increases the sensitivity of colorectal cancer to 5-fluorouracil treatment by inducing apoptosis and autophagic cell death. Signal Transduct Target Ther 4:53 (2019). PubMed: 31815010
    • Luo M  et al. MiR-196b affects the progression and prognosis of human LSCC through targeting PCDH-17. Auris Nasus Larynx N/A:N/A (2018). PubMed: 30454973

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