Overview

  • Product name

    Anti-PCDH7 antibody [OTI2G6]
    See all PCDH7 primary antibodies
  • Description

    Mouse monoclonal [OTI2G6] to PCDH7
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human PCDH7 (amino acids 1-1247) produced in HEK293T cells (NP_115833).

  • Positive control

    • HEK293T cell lysate transfected with pCMV6-ENTRY PCDH7; HepG2, HeLa, SVT2, COS7, MDCK and MCF7 cell extracts; Human endometrium tissue; COS7 cells transiently transfected with pCMV6-ENTRY PCDH7
  • General notes

    The clone number has been updated from 2G6 to OTI2G6, both clone numbers name the same clone.

    Dilute in PBS (pH7.3) before use.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab139274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 116 kDa.
IHC-P 1/150.
ICC/IF 1/100.

Target

  • Tissue specificity

    Expressed predominantly in brain and heart and at lower levels in various other tissues.
  • Sequence similarities

    Contains 7 cadherin domains.
  • Post-translational
    modifications

    Isoform C is phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BH Pcdh antibody
    • BH protocadherin antibody
    • BH-Pcdh antibody
    • BHPCDH antibody
    • Brain heart protocadherin antibody
    • Brain-heart protocadherin antibody
    • PCDH 7 antibody
    • PCDH7 antibody
    • PCDH7_HUMAN antibody
    • Protocadherin 7 antibody
    • Protocadherin-7 antibody
    see all

Images

  • All lanes : Anti-PCDH7 antibody [OTI2G6] (ab139274) at 1/200 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PCDH7

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 116 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206769)
  • All lanes : Anti-PCDH7 antibody [OTI2G6] (ab139274) at 1/200 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 116 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206740)
  • Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labelling PCDH7 with ab139274 at 1/150 dilution.
  • Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY PCDH7 using ab139274 at 1/100 dilution.

References

This product has been referenced in:

  • Xiao H  et al. Overexpression of protocadherin 7 inhibits neuronal survival by downregulating BIRC5 in vitro. Exp Cell Res 366:71-80 (2018). WB ; Mouse . Read more (PubMed: 29548751) »
  • Coll M  et al. Generation of Hepatic Stellate Cells from Human Pluripotent Stem Cells Enables In Vitro Modeling of Liver Fibrosis. Cell Stem Cell 23:101-113.e7 (2018). Read more (PubMed: 30049452) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (kidney cortex, cerebral cortex)
Gel Running Conditions
Reduced Denaturing (SDS-PAGE (10% Acrylamide))
Loading amount
20 µg
Specification
kidney cortex, cerebral cortex
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Oct 18 2016

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