Western blot abreview for Anti-PCDH7 antibody [2G6]

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (kidney cortex, cerebral cortex)
Gel Running Conditions
Reduced Denaturing (SDS-PAGE (10% Acrylamide))
Loading amount
20 µg
Specification
kidney cortex, cerebral cortex
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Mouse EnVision
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
anti-KLH antibody were used as a primery antibody of negative control group.
Exposure
5 second(s)
Bands
Specific: 148 kDa

Additional data

Additional Notes
1. kidney cortex 2. cerebral cortex

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Submitted Oct 18 2016

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