Anti-PCDHA1 antibody (ab231152)
Key features and details
- Rabbit polyclonal to PCDHA1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCDHA1 antibody -
Description
Rabbit polyclonal to PCDHA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human PCDHA1 aa 1-250. Expressed in E. coli.
Database link: Q9Y5I3-1 -
Positive control
- IHC-P: Human cerebrum tissue. WB: Recombinant human PCDHA1 protein; Human serum.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 103 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 103 kDa. |
Target
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Relevance
PCDHA1 (Protocadherin alpha 1) is a member of the protocadherin family, a subgroup of the cadherin superfamily. Protocadherins are neural cadherin-like cell adhesion proteins that are integral membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific neuronal connections in the brain. PCDHA1 is one of fifteen tandemly arranged genes within the alpha cluster of protocadherin genes on 5q31. -
Cellular localization
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. -
Database links
- Entrez Gene: 56147 Human
- Omim: 606307 Human
- SwissProt: Q9Y5I3 Human
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Alternative names
- KIAA0345 like 13 antibody
- PCDH alpha1 antibody
- PCDHA 1 antibody
- Protocadherin alpha 1 antibody
Images
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PCDHA1 with ab231152 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-PCDHA1 antibody (ab231152) at 1 µg/ml + Human serum
Predicted band size: 103 kDa -
Anti-PCDHA1 antibody (ab231152) at 2 µg/ml + Recombinant human PCDHA1 protein
Predicted band size: 103 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231152 has not yet been referenced specifically in any publications.