Overview

  • Product name
    Anti-PCDHB14 antibody - N-terminal
  • Description
    Rabbit polyclonal to PCDHB14 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PCDHB14 aa 165-193 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9Y5E9

  • Positive control
    • K562 cell line lysate, Human brain tissue, K562 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 88 kDa.

Target

  • Function
    Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
  • Sequence similarities
    Contains 6 cadherin domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC120065 antibody
    • PCDBE_HUMAN antibody
    • PCDH beta14 antibody
    • PCDH-beta-14 antibody
    • PCDHB 14 antibody
    • PCDHB14 antibody
    • Protocadherin beta 14 antibody
    • Protocadherin beta-14 antibody
    see all

Images

  • Flow Cytometrical analysis of K562  cells with ab170689 antibody at a dilution of 1/10 (right histogram) compared to a negative control cell (left histogram).

  • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling PCDHB14 with ab170689 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-PCDHB14 antibody - N-terminal (ab170689) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size: 88 kDa

References

ab170689 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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