Key features and details
- Rabbit polyclonal to PCF11
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PCF11 antibody
See all PCF11 primary antibodies
DescriptionRabbit polyclonal to PCF11
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 1505-1555 of Human PCF11 (NP_056969.2).
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab134391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 173 kDa.|
|IP||Use a concentration of 2 - 10 µg/ml.|
FunctionComponent of pre-mRNA cleavage complex II.
Sequence similaritiesContains 1 CID domain.
- Information by UniProt
- hPCF11 antibody
- KIAA0824 antibody
- OTTHUMP00000234826 antibody
All lanes : Anti-PCF11 antibody (ab134391) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 173 kDa
Exposure time: 30 seconds
Detection of PCF11 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab134391 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 seconds.
ab134391 has been referenced in 1 publication.
- Volanakis A et al. WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription. Genes Dev 31:2175-2185 (2017). PubMed: 29196535