Overview

  • Product name

  • Description

    Rabbit polyclonal to PCF11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1505-1555 of Human PCF11 (NP_056969.2).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899)

Properties

Applications

Our Abpromise guarantee covers the use of ab134391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 173 kDa.
IP Use a concentration of 2 - 10 µg/ml.

Target

  • Function

    Component of pre-mRNA cleavage complex II.
  • Sequence similarities

    Contains 1 CID domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPCF11 antibody
    • KIAA0824 antibody
    • OTTHUMP00000234826 antibody
    • PCF11 antibody
    • PCF11 cleavage and polyadenylation factor subunit antibody
    • PCF11, cleavage and polyadenylation factor II subunit, homolog antibody
    • PCF11, cleavage and polyadenylation factor subunit, homolog (S. antibody
    • PCF11_HUMAN antibody
    • PCF11p homolog antibody
    • Pre-mRNA cleavage complex 2 protein Pcf11 antibody
    • Pre-mRNA cleavage complex II protein Pcf11 antibody
    see all

Images

  • All lanes : Anti-PCF11 antibody (ab134391) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 173 kDa


    Exposure time: 30 seconds
  • Detection of PCF11 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab134391 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 3 seconds.

References

This product has been referenced in:

  • Volanakis A  et al. WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription. Genes Dev 31:2175-2185 (2017). Read more (PubMed: 29196535) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134391.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up