Overview

  • Product name

    Anti-PCGF3+PCGF5 antibody [EPR19475]
    See all PCGF3+PCGF5 primary antibodies
  • Description

    Rabbit monoclonal [EPR19475] to PCGF3+PCGF5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse PCGF3+PCGF5 aa 100-200. The exact sequence is proprietary.
    Database link: Q8BTQ0

  • Positive control

    • WB: Wildtype mouse embryonic stem cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

Images

  • All lanes : Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510) at 1/1000 dilution

    Lane 1 : PCGF3 WT mouse embryonic stem cell lysate
    Lane 2 : PCGF3 knockout mouse embryonic stem cell lysate
    Lane 3 : PCGF3/PCGF5 knockout mouse embryonic stem cell lysate

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG-HRP at 1/2000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    Blocking buffer: 3% milk in PBST

    Dilution buffer: 3% BSA in PBST.

    Exposure time: 2-10 minutes.

    The data is kindly provided by our collaborator Dr. Jinzhong Qin (Nanjing University).

References

This product has been referenced in:

  • Zhao W  et al. Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity. J Biol Chem 292:2773-2784 (2017). WB . Read more (PubMed: 28049731) »
See 1 Publication for this product

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