Product nameAnti-PCGF3+PCGF5 antibody [EPR19475] - BSA and Azide free
See all PCGF3+PCGF5 primary antibodies
DescriptionRabbit monoclonal [EPR19475] to PCGF3+PCGF5 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse PCGF3+PCGF5 aa 100-200. The exact sequence is proprietary.
Database link: Q8BTQ0
- WB: Wildtype mouse embryonic stem cell lysate.
Ab222922 is the carrier-free version of ab201510. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab222922 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
RelevanceComponent of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility
Cellular localizationPCGF3: Nucleus.
All lanes : Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510) at 1/1000 dilution
Lane 1 : PCGF3 WT mouse embryonic stem cell lysate
Lane 2 : PCGF3 knockout mouse embryonic stem cell lysate
Lane 3 : PCGF3/PCGF5 knockout mouse embryonic stem cell lysate
Lysates/proteins at 100 µg per lane.
All lanes : Goat anti-rabbit IgG-HRP at 1/2000 dilution
Predicted band size: 28 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222922).
Blocking buffer: 3% milk in PBST
Dilution buffer: 3% BSA in PBST.
Exposure time: 2-10 minutes.
This product has been referenced in:
- Zhao W et al. Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity. J Biol Chem 292:2773-2784 (2017). WB . Read more (PubMed: 28049731) »