Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19475] to PCGF3+PCGF5 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Product nameAnti-PCGF3+PCGF5 antibody [EPR19475] - BSA and Azide free
See all PCGF3+PCGF5 primary antibodies
DescriptionRabbit monoclonal [EPR19475] to PCGF3+PCGF5 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse PCGF3+PCGF5 aa 100-200. The exact sequence is proprietary.
Database link: Q8BTQ0
- WB: Wildtype mouse embryonic stem cell lysate.
Ab222922 is the carrier-free version of ab201510. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab222922 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
RelevanceComponent of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility
Cellular localizationPCGF3: Nucleus.
All lanes : Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510) at 1/1000 dilution
Lane 1 : PCGF3 WT mouse embryonic stem cell lysate
Lane 2 : PCGF3 knockout mouse embryonic stem cell lysate
Lane 3 : PCGF3/PCGF5 knockout mouse embryonic stem cell lysate
Lysates/proteins at 100 µg per lane.
All lanes : Goat anti-rabbit IgG-HRP at 1/2000 dilution
Predicted band size: 28 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222922).
Blocking buffer: 3% milk in PBST
Dilution buffer: 3% BSA in PBST.
Exposure time: 2-10 minutes.
ab222922 has been referenced in 1 publication.
- Zhao W et al. Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity. J Biol Chem 292:2773-2784 (2017). WB . PubMed: 28049731