Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-PCGF3+PCGF5 antibody [EPR19475] - BSA and Azide free (ab222922)

Overview

  • Product name

    Anti-PCGF3+PCGF5 antibody [EPR19475] - BSA and Azide free
    See all PCGF3+PCGF5 primary antibodies
  • Description

    Rabbit monoclonal [EPR19475] to PCGF3+PCGF5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse PCGF3+PCGF5 aa 100-200. The exact sequence is proprietary.
    Database link: Q8BTQ0

  • Positive control

    • WB: Wildtype mouse embryonic stem cell lysate.
  • General notes

    Ab222922 is the carrier-free version of ab201510. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab222922 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab222922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

Images

  • All lanes : Anti-PCGF3+PCGF5 antibody [EPR19475] (ab201510) at 1/1000 dilution

    Lane 1 : PCGF3 WT mouse embryonic stem cell lysate
    Lane 2 : PCGF3 knockout mouse embryonic stem cell lysate
    Lane 3 : PCGF3/PCGF5 knockout mouse embryonic stem cell lysate

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG-HRP at 1/2000 dilution

    Predicted band size: 28 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222922).

    Blocking buffer: 3% milk in PBST

    Dilution buffer: 3% BSA in PBST.

    Exposure time: 2-10 minutes.

References

This product has been referenced in:

  • Zhao W  et al. Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity. J Biol Chem 292:2773-2784 (2017). WB . Read more (PubMed: 28049731) »
See 1 Publication for this product

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