Overview

  • Product name

    Anti-PCGF5 antibody [EP19476]
    See all PCGF5 primary antibodies
  • Description

    Rabbit monoclonal [EP19476] to PCGF5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse PCGF5 aa 1-100. The exact sequence is proprietary.
    Database link: Q3UK78

  • Positive control

    • WB: Mouse testis, ovary and thymus lysates; mES-D3, F9, C6, RAW 264.7, PC-12, NIH/3T3 and HeLa whole cell lysates; human and rat testis lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

Images

  • All lanes : Anti-PCGF5 antibody [EP19476] (ab201511) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Mouse ovary lysate
    Lane 3 : Mouse thymus lysate
    Lane 4 : mES-D3 (Mouse embryo stem cell line) whole cell lysate
    Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-PCGF5 antibody [EP19476] (ab201511) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 5 : Rat testis lysate
    Lane 6 : Human testis lysate
    Lane 7 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 30 seconds; Lane 5-7: 3 minutes.

References

This product has been referenced in:

  • Zhao W  et al. Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity. J Biol Chem 292:2773-2784 (2017). WB . Read more (PubMed: 28049731) »
  • Yan Y  et al. Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells. Sci Rep 7:46276 (2017). Read more (PubMed: 28393894) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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