Overview

  • Product name

    Anti-PCGF6 antibody [EPR19450]
    See all PCGF6 primary antibodies
  • Description

    Rabbit monoclonal [EPR19450] to PCGF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse PCGF6 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99NA9

  • Positive control

    • WB: Mouse testis, thymus, ovary and embryo lysates; F9, C6, RAW 264.7 and PC-12 whole cell lysates; rat testis lysate. ICC/IF: F9 cells. IP: F9 whole cell lysate.
  • General notes

     

     

     This product was previously labelled as RING6a

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab200038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa).
ICC/IF 1/100.

ICC is recommended for mouse only.

IP 1/30.

Target

  • Function

    Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated during mitosis. Phosphorylated on Ser-30 by CDK7 in vitro.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • MBLR antibody
    • Mel18 and Bmi1 like RING finger protein antibody
    • Mel18 and Bmi1-like RING finger antibody
    • Pcgf6 antibody
    • PCGF6_HUMAN antibody
    • Polycomb group RING finger protein 6 antibody
    • RING finger protein 134 antibody
    • RNF134 antibody
    see all

Images

  • All lanes : Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Mouse thymus lysate
    Lane 3 : Mouse ovary lysate
    Lane 4 : Mouse embryo lysate
    Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 3 minutes; Lane 5: 10 seconds.

  • All lanes : Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

    Lane 1 : Rat testis lysate
    Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2 : 3 minutes; Lane 3/4: 30 seconds.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic testicular cancer cell line) cells labeling PCGF6 with ab200038 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on F9 cells. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab200038 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary  antibody at 1/1000 dilution.

  • Ab200038 Immunoprecipitating PCGF6 in human F9(mouse embryonal carcinoma) whole cell lysate. 350µg of cell lysate was incubated with primary antibody (2 µg/ml). For western blotting Ab200038 (1/1000) was used to confirm successful immunoprecipation. For detection VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000.

    Lane 1: (Input) F9(mouse embryonal carcinoma) whole cell lysate 10μg
    Lane 2: (+) F9 whole cell lysate
    Lane 3: (-) Rabbit monoclonal IgG (ab172730) instead of ab200038 in F9 whole cell lysate

References

ab200038 has not yet been referenced specifically in any publications.

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