Key features and details
- Rabbit polyclonal to PCID1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
Product nameAnti-PCID1 antibody
See all PCID1 primary antibodies
DescriptionRabbit polyclonal to PCID1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Orangutan, Xenopus tropicalis
Recombinant full length protein corresponding to Human PCID1 aa 1-374.
MSVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACD VCLKEDDKDVESVMNSVVSLLLILEPDKQEALIESLCEKLVKFREGERPS LRLQLLSNLFHGMDKNTPVRYTVYCSLIKVAASCGAIQYIPTELDQVRKW ISDWNLTTEKKHTLLRLLYEALVDCKKSDAASKVMVELLGSYTEDNASQA RVDAHRCIVRALKDPNAFLFDHLLTLKPVKFLEGELIHDLLTIFVSAKLA SYVKFYQNNKDFIDSLGLLHEQNMAKMRLLTFMGMAVENKEISFDTMQQE LQIGADDVEAFVIDAVRTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQL YDTLNAWKQNLNKVKNSLLSLSDT
Database link: Q7L2H7
- WB: HeLa and A549 whole cell lysates; Human placenta tissue lysate; Mouse gonadal, stomach and brain tissue lysates. IP: HeLa whole cell lysate. IHC-P: Human pancreatic cancer and testis tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 28,43 kDa).|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).
Tissue specificityBroadly expressed.
Sequence similaritiesBelongs to the eIF-3 subunit M family.
Contains 1 PCI domain.
- Information by UniProt
- B5 antibody
- B5 receptor antibody
- Dendritic cell protein antibody
All lanes : Anti-PCID1 antibody (ab230105) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : Mouse gonadal tissue lysate
Lane 4 : Human placenta tissue lysate
Lane 5 : Mouse stomach tissue lysate
Lane 6 : Mouse brain tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28,43 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Paraffin-embedded human pancreatic cancer tissue stained for PCID1 using ab230105 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for PCID1 using ab230105 at 1/100 dilution in immunohistochemical analysis.
PCID1 was immunoprecipitated from 0.5 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg of ab230105. Western blot was performed from the immunoprecipitate using ab230105. HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.
Lane 1: 1 µg control IgG instead of ab230105 in HeLa whole cell lysate .
Lane 2: ab230105 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230105 has not yet been referenced specifically in any publications.