Key features and details
- Rabbit polyclonal to PCID1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-PCID1 antibody
See all PCID1 primary antibodies
DescriptionRabbit polyclonal to PCID1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Cow, Orangutan
Recombinant full length protein corresponding to Human PCID1 aa 2-374.
SVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACDV CLKEDDKDVESVMNSVVSLLLILEPDKQEALIESLCEKLVKFREGERPSL RLQLLSNLFHGMDKNTPVRYTVYCSLIKVAASCGAIQYIPTELDQVRKWI SDWNLTTEKKHTLLRLLYEALVDCKKSDAASKVMVELLGSYTEDNASQAR VDAHRCIVRALKDPNAFLFDHLLTLKPVKFLEGELIHDLLTIFVSAKLAS YVKFYQNNKDFIDSLGLLHEQNMAKMRLLTFMGMAVENKEISFDTMQQEL QIGADDVEAFVIDAVRTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQLY DTLNAWKQNLNKVKNSLLSLSDT
Database link: Q7L2H7
- WB: HeLa, HepG2 and A549 whole cell lysates; Rat heart and spleen lysates. IHC-P: Human testis and pancreatic cancer tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%
Our Abpromise guarantee covers the use of ab237504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 43 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).
Tissue specificityBroadly expressed.
Sequence similaritiesBelongs to the eIF-3 subunit M family.
Contains 1 PCI domain.
- Information by UniProt
- B5 antibody
- B5 receptor antibody
- Dendritic cell protein antibody
All lanes : Anti-PCID1 antibody (ab237504) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat spleen lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Paraffin-embedded human testis tissue stained for PCID1 using ab237504 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreatic cancer tissue stained for PCID1 using ab237504 at 1/100 dilution in immunohistochemical analysis.
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for PCID1 (green) using ab237504 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237504 has not yet been referenced specifically in any publications.