Overview

  • Product name

    Anti-PCIF1 antibody
  • Description

    Rabbit polyclonal to PCIF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cat, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human PCIF1 aa 654-704 (C terminal). The exact sequence is proprietary. NP_071387.1.
    Sequence:

    APTPERL QELSAAYRQS GRSHSSGSSS SSSSEAKDRD SGREQGPSRE PHPT


    Database link: Q9H4Z3

  • Positive control

    • Jurkat, HeLa, NIH 3T3, 293T and TCMK1 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab205016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/5000. Predicted molecular weight: 81 kDa.

Target

Images

  • Detection of PCIF1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205016 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab205016 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.

  • All lanes : Anti-PCIF1 antibody (ab205016) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 81 kDa


    Exposure time: 3 minutes

References

ab205016 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205016.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up