Recombinant Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free (ab281938)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24319-21] to PCIF1 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PCIF1 antibody [EPR24319-21] - BSA and Azide free
See all PCIF1 primary antibodies -
Description
Rabbit monoclonal [EPR24319-21] to PCIF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T, MCF-7, HeLa, Jurkat, Human testis and Rat liver, Rat testis lysates. IP: MCF-7 cells.
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General notes
ab281938 is the carrier-free version of ab271081.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24319-21 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa. |
Target
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Function
May play a role in transcription elongation or in coupling transcription to pre-mRNA processing through its association with the phosphorylated C-terminal domain (CTD) of RNAPII largest subunit. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 WW domain. -
Domain
The WW domain is sufficient for direct and specific interaction with the phosphorylated CTD of RNAPII largest subunit. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 63935 Human
- Entrez Gene: 362269 Rat
- SwissProt: Q9H4Z3 Human
- Unigene: 472856 Human
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Alternative names
- bA465L10.1 antibody
- C20orf67 antibody
- Pcif1 antibody
see all
Images
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All lanes : Anti-PCIF1 antibody [EPR24319-21] (ab271081) at 1/1000 dilution
Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?This data was developed using 271081, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot (lane 3 and 4) was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
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Anti-PCIF1 antibody [EPR24319-21] (ab271081) at 1/1000 dilution + Human testis tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?This data was developed using ab271081, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes.
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All lanes : Anti-PCIF1 antibody [EPR24319-21] (ab271081) at 1/1000 dilution
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?This data was developed using ab271081, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes.
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This data was developed using ab271081, the same antibody clone in a different buffer formulation.
PCIF1 was immunoprecipitated from 0.35 mg MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab271081 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271081 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab271081 IP in MCF-7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271081 in MCF-7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
This blot was developed using a higher sensitivity ECL substrate.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab281938 has not yet been referenced specifically in any publications.