Overview

  • Product name
  • Description
    Rabbit polyclonal to PCK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, Monkey
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Caenorhabditis elegans
  • Immunogen

    Synthetic peptide derived from within residues 330 - 379 of human PEP Carboxylase.

  • Positive control
    • Jurkat cell lysate, Human kidney

Applications

Our Abpromise guarantee covers the use of ab28455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • Tissue specificity
    Major sites of expression are liver, kidney and adipocytes.
  • Pathway
    Carbohydrate biosynthesis; gluconeogenesis.
  • Involvement in disease
    Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • Sequence similarities
    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • Post-translational
    modifications
    Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytosolic [GTP] antibody
    • GTP antibody
    • PCK1 antibody
    • PCKGC_HUMAN antibody
    • PEP carboxykinase antibody
    • PEPCK-C antibody
    • PEPCK1 antibody
    • PEPCKC antibody
    • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
    • Phosphoenolpyruvate carboxykinase 1 antibody
    • Phosphoenolpyruvate carboxykinase antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Images

  • Western Blot using ab28455 at 1.0ug/ml. Sample type: Hum. Fetal Heart Lysate
  • Western Blot using ab28455 at 1.0ug/ml. Sample type: Hum. Fetal Liver Lysate
  • Western Blot using ab28455 at 1.0ug/ml. Sample type: Hum. Fetal Lung Lysate
  • Western Blot using ab28455 at 1.0ug/ml. Sample type: Hum. Fetal Muscle Lysate
  • Western Blot using ab28455 at 1.0ug/ml. Sample type: Human MCF7 cell Lysate
  • Western Blot using ab28455 at 1.0ug/ml. Sample type: Human Jurkat cell Lysate
  • Anti-PCK1 antibody (ab28455) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1:50,000-100,000

    Predicted band size: 69 kDa

  • Antibody concentration: 4 - 8 ug/ml Paraffin-embeded Tissue: Human Kidney Cells with positive label: Epithelial cells of renal tubule (indicated with arrows) Magnification: x400
  • ICC/IF image of ab28455 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28455, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Latorre P  et al. O-GlcNAcylation mediates the control of cytosolic phosphoenolpyruvate carboxykinase activity via Pgc1a. PLoS One 12:e0179988 (2017). Read more (PubMed: 28644880) »
  • Sun X  et al. Comparative proteomic profiling identifies potential prognostic factors for human clear cell renal cell carcinoma. Oncol Rep 36:3131-3138 (2016). WB ; Human . Read more (PubMed: 27748938) »
See all 10 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Sheep Tissue lysate - whole (Fetal and Maternal Liver)
Loading amount
100 µg
Specification
Fetal and Maternal Liver
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% Tris/Glycine-SDS)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Rob Cordery-Cotter

Verified customer

Submitted Nov 21 2012

Question
Answer

Thank you for your phone call.

DISCOUNT CODE: xxx for ab28455, Value: xxx
DISCOUNT CODE: xxx for ab83690, Value: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab28455 and ab83690 in mouse. Each code will give you xxx and xxx, respectively, off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Read More

Question
Answer

I am very pleased to hear you would like to test ab28455, ab83690, and ab138003 in sheep. Each code will give you for value listed above off a future order. To redeem this offer, please submit an Abreview for sheep for each antibody and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Read More

Answer

Merci de votre intérêt pour les anticorps Abcam. Oui, je recommanderais ab37796 pour le marquage de la glucokinase et ab69508 pour le récepteur insuline beta. Par contre, pour le marquage de PCK1 je recommanderais plutôt un anticorps testé en IHC-P tel que ab28455 (www.abcam.com/ab28455) ou ab115693 (www.abcam.com/ab115693). Concernant les deux anti-PCK1, à ma connaissance ces anticorps n’ont pas encore été testés chez la souris et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab28455 ou ab115693 chez la souris, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab28455 ou ab115693 pourra être utilisable lors d’une prochaine commande d’anticorps primaire. Etapes à suivre : 1. Nous confirmer que vous souhaiteriez tester ab28455 ou ab115693 chez la souris afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab28455 ou ab115693. 2. Acheter ab28455 ou ab115693 par téléphone, fax ou internet (www.abcam.com) 3. Le tester chez la souris 4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews 5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire. Même si ab28455 ou ab115693 ne pas fonctionne pas chez la souris, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps. N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle. Termes et Conditions : www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Monkey Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Hepatoma cell line (H4IIE))
Loading amount
50 µg
Specification
Hepatoma cell line (H4IIE)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Prof. Jianping Ye

Verified customer

Submitted Mar 29 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (hepatocytes (HepG2))
Loading amount
50 µg
Specification
hepatocytes (HepG2)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Prof. Jianping Ye

Verified customer

Submitted Mar 29 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up