Overview

  • Product name

  • Description

    Rabbit polyclonal to PCK1/PEPC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PCK1/PEPC aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab86626)

  • Positive control

    • Recombinant Human PCK1/PEPC protein (ab119469) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: HepG2 Whole Cell Lysate Rat Liver Tissue Lysate Mouse Liver Tissue Lysate Human Liver Tissue Lysate - adult normal tissue
  • General notes

     This product was previously labelled as PCK1

     

Applications

Our Abpromise guarantee covers the use of ab70358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

Abcam recommends using milk as the blocking agent.

Target

  • Function

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • Tissue specificity

    Major sites of expression are liver, kidney and adipocytes.
  • Pathway

    Carbohydrate biosynthesis; gluconeogenesis.
  • Involvement in disease

    Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • Sequence similarities

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • Post-translational
    modifications

    Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • cytosolic [GTP] antibody
    • GTP antibody
    • PCK1 antibody
    • PCKGC_HUMAN antibody
    • PEP carboxykinase antibody
    • PEPCK-C antibody
    • PEPCK1 antibody
    • PEPCKC antibody
    • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
    • Phosphoenolpyruvate carboxykinase 1 antibody
    • Phosphoenolpyruvate carboxykinase antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Images

  • All lanes : Anti-PCK1/PEPC antibody (ab70358) at 1 µg

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate
    Lane 4 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 69 kDa
    Additional bands at: 32 kDa, 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab70358 overnight at 4°C. Antibody binding was detected using an anti-rabbit VHH single domain antibody conjugated to HRP, and visualised using ECL development solution ab133406.

     

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • All lanes : Anti-PCK1/PEPC antibody (ab70358) at 1 µg/ml

    All lanes : Mouse adipose tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 69 kDa


    Exposure time: 5 hours

    See Abreview

References

This product has been referenced in:

  • Sharma AX  et al. Glucagon Receptor Antagonism Improves Glucose Metabolism and Cardiac Function by Promoting AMP-Mediated Protein Kinase in Diabetic Mice. Cell Rep 22:1760-1773 (2018). WB ; Mouse . Read more (PubMed: 29444429) »
  • Wang Z  et al. Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice. Mol Cell Endocrinol 474:127-136 (2018). WB ; Mouse . Read more (PubMed: 29499209) »
See all 17 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver Sections from C57Bl mice)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Liver Sections from C57Bl mice
Blocking step
Innovex background buster as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 22 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver Extract)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin)
Loading amount
20 µg
Specification
Liver Extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 25 2017

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Tissue lysate - whole (Adipose tissue lysate)
Specification
Adipose tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 27 2013

Answer

Merci de votre intérêt pour les anticorps Abcam. Oui, je recommanderais ab37796 pour le marquage de la glucokinase et ab69508 pour le récepteur insuline beta. Par contre, pour le marquage de PCK1 je recommanderais plutôt un anticorps testé en IHC-P tel que ab28455 (www.abcam.com/ab28455) ou ab115693 (www.abcam.com/ab115693). Concernant les deux anti-PCK1, à ma connaissance ces anticorps n’ont pas encore été testés chez la souris et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab28455 ou ab115693 chez la souris, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab28455 ou ab115693 pourra être utilisable lors d’une prochaine commande d’anticorps primaire. Etapes à suivre : 1. Nous confirmer que vous souhaiteriez tester ab28455 ou ab115693 chez la souris afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab28455 ou ab115693. 2. Acheter ab28455 ou ab115693 par téléphone, fax ou internet (www.abcam.com) 3. Le tester chez la souris 4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews 5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire. Même si ab28455 ou ab115693 ne pas fonctionne pas chez la souris, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps. N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle. Termes et Conditions : www.abcam.com/collaborationdiscount.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - other (Liver, cytosol)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Liver, cytosol
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Werner Kovacs

Verified customer

Submitted Sep 30 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up