• Product name

  • Description

    Rabbit polyclonal to PCK1/PEPC
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Horse, Chicken, Cow, Pig, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PCK1/PEPC aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab217264)

  • Positive control

    • Recombinant Human PCK1/PEPC protein (ab114362) can be used as a positive control in WB. This antibody gave a positive signal in the following tissue lysates: Human Liver Rat Liver Mouse Liver
  • General notes

     This product was previously labelled as PCK1



Our Abpromise guarantee covers the use of ab87340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).Can be blocked with PCK1/PEPC peptide (ab217264).


  • Function

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • Tissue specificity

    Major sites of expression are liver, kidney and adipocytes.
  • Pathway

    Carbohydrate biosynthesis; gluconeogenesis.
  • Involvement in disease

    Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • Sequence similarities

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • Post-translational

    Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • cytosolic [GTP] antibody
    • GTP antibody
    • PCK1 antibody
    • PCKGC_HUMAN antibody
    • PEP carboxykinase antibody
    • PEPCK-C antibody
    • PEPCK1 antibody
    • PEPCKC antibody
    • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
    • Phosphoenolpyruvate carboxykinase 1 antibody
    • Phosphoenolpyruvate carboxykinase antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all


  • All lanes : Anti-PCK1/PEPC antibody (ab87340) at 2 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

    Exposure time: 15 minutes


This product has been referenced in:

  • Ma X  et al. Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes Nutr 14:23 (2019). Read more (PubMed: 31367261) »
  • Wang HY  et al. AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues. Biochem J 449:479-89 (2013). Read more (PubMed: 23078342) »
See all 2 Publications for this product

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