Overview

  • Product name

    Anti-PCK1/PEPC antibody [EPR6939]
    See all PCK1/PEPC primary antibodies
  • Description

    Rabbit monoclonal [EPR6939] to PCK1/PEPC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PCK1/PEPC aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney and Human adipose tissue lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as PCK1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab125003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
    • Tissue specificity

      Major sites of expression are liver, kidney and adipocytes.
    • Pathway

      Carbohydrate biosynthesis; gluconeogenesis.
    • Involvement in disease

      Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
    • Sequence similarities

      Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
    • Post-translational
      modifications

      Acetylation is increased on addition of glucose and appears to regulate the protein stability.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • cytosolic [GTP] antibody
      • GTP antibody
      • PCK1 antibody
      • PCKGC_HUMAN antibody
      • PEP carboxykinase antibody
      • PEPCK-C antibody
      • PEPCK1 antibody
      • PEPCKC antibody
      • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
      • Phosphoenolpyruvate carboxykinase 1 antibody
      • Phosphoenolpyruvate carboxykinase antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic antibody
      • Phosphoenolpyruvate carboxylase antibody
      • Phosphopyruvate carboxylase antibody
      see all

    Images

    • All lanes : Anti-PCK1/PEPC antibody [EPR6939] (ab125003) at 1/1000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Human adipose tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 69 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab125003 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab125003.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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