Product namePCK1/PEPC peptide
See all PCK1/PEPC proteins and peptides
Purity> 90 % n/a.
Our Abpromise guarantee covers the use of ab217264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-PCK1/PEPC antibody (ab87340)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
This product was previously labelled as PCK1
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C.
- cytosolic [GTP]
FunctionCatalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Tissue specificityMajor sites of expression are liver, kidney and adipocytes.
PathwayCarbohydrate biosynthesis; gluconeogenesis.
Involvement in diseaseDefects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
Sequence similaritiesBelongs to the phosphoenolpyruvate carboxykinase [GTP] family.
modificationsAcetylation is increased on addition of glucose and appears to regulate the protein stability.
- Information by UniProt
ab217264 has not yet been referenced specifically in any publications.