Overview

  • Product name

  • Description

    Rabbit polyclonal to PCK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 10-243 of Human PCK2

  • Positive control

    • HepG2, HCT116, Neuro2A, GL261 and PC-12 cells. Breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • Pathway

    Carbohydrate biosynthesis; gluconeogenesis.
  • Involvement in disease

    Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • Sequence similarities

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 4.1.1.32 antibody
    • GTP mitochondrial precursor antibody
    • HGNC:8725 antibody
    • mitochondrial antibody
    • Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody
    • OTTHUMP00000164700 antibody
    • PCK2 antibody
    • PCKGM_HUMAN antibody
    • PE antibody
    • PEP carboxykinase antibody
    • PEPCK antibody
    • PEPCK deficiency mitochondrial antibody
    • PEPCK M antibody
    • PEPCK-M antibody
    • PEPCK2 antibody
    • Phosphoenolpyruvate carboxykinase [GTP] antibody
    • Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody
    • Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Images

  • All lanes : Anti-PCK2 antibody (ab137580) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 71 kDa



    7.5% SDS-PAGE
  • Anti-PCK2 antibody (ab137580) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size: 71 kDa



    7.5% SDS-PAGE
  • All lanes : Anti-PCK2 antibody (ab137580) at 1/1000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 71 kDa



    7.5% SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labelling PCK2 with ab137580 at 1/250 dilution.

References

This product has been referenced in:

  • Leithner K  et al. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24632615) »
See 1 Publication for this product

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