Anti-PCK2 antibody (ab137580)
Key features and details
- Rabbit polyclonal to PCK2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PCK2 antibody
See all PCK2 primary antibodies -
Description
Rabbit polyclonal to PCK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 10-243 of Human PCK2
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Positive control
- HepG2, HCT116, Neuro2A, GL261 and PC-12 cells. Breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 71 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 71 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. -
Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Involvement in disease
Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. -
Sequence similarities
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 5106 Human
- Entrez Gene: 74551 Mouse
- Entrez Gene: 361042 Rat
- Omim: 261650 Human
- SwissProt: Q16822 Human
- SwissProt: Q8BH04 Mouse
- Unigene: 75812 Human
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Alternative names
- EC 4.1.1.32 antibody
- GTP mitochondrial precursor antibody
- HGNC:8725 antibody
see all
Images
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Western blot - Anti-PCK2 antibody (ab137580)All lanes : Anti-PCK2 antibody (ab137580) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
7.5% SDS-PAGE -
Western blot - Anti-PCK2 antibody (ab137580)Anti-PCK2 antibody (ab137580) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 71 kDa
7.5% SDS-PAGE -
Western blot - Anti-PCK2 antibody (ab137580)All lanes : Anti-PCK2 antibody (ab137580) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
7.5% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody (ab137580)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling PCK2 with ab137580 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137580 has been referenced in 2 publications.
- Strickaert A et al. Reprogramming of Energy Metabolism: Increased Expression and Roles of Pyruvate Carboxylase in Papillary Thyroid Cancer. Thyroid 29:845-857 (2019). PubMed: 30990120
- Leithner K et al. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer. Oncogene N/A:N/A (2014). Human . PubMed: 24632615