Overview

  • Product name

  • Description

    Rabbit polyclonal to PCM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human PCM1 (internal sequence). The exact sequence is proprietary.
    Database link: Q15154

  • Positive control

    • WB: Jurkat, Raji and NCI-H929 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 229 kDa.

Target

  • Function

    Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.
  • Tissue specificity

    Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.
  • Involvement in disease

    Defects in PCM1 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving PCM1 is found in thyroid papillary carcinomas. Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1.
    Note=A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma. Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1.
  • Sequence similarities

    Belongs to the PCM1 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic granule. Localizes to cytoplasmic granules which are enriched around the centrosome. This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPCM-1 antibody
    • PCM-1 antibody
    • pcm1 antibody
    • PCM1_HUMAN antibody
    • Pericentriolar material 1 antibody
    • Pericentriolar material 1 protein antibody
    • PTC4 antibody
    see all

Images

  • All lanes : Anti-PCM1 antibody (ab229637) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell extracts
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell extracts
    Lane 3 : NCI-H929 whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 229 kDa



    5% SDS-PAGE

References

ab229637 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229637.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up