Anti-PCM1 antibody [CL0206] (ab154142)
Key features and details
- Mouse monoclonal [CL0206] to PCM1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PCM1 antibody [CL0206]
See all PCM1 primary antibodies -
Description
Mouse monoclonal [CL0206] to PCM1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis, cerebral cortex, fallopian tube and striated muscle tissues; RT4 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL0206 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 229 kDa.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 229 kDa. |
Target
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Function
Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia. -
Tissue specificity
Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid. -
Involvement in disease
Defects in PCM1 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving PCM1 is found in thyroid papillary carcinomas. Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1.
Note=A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma. Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1. -
Sequence similarities
Belongs to the PCM1 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic granule. Localizes to cytoplasmic granules which are enriched around the centrosome. This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase. - Information by UniProt
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Database links
- Entrez Gene: 5108 Human
- Entrez Gene: 18536 Mouse
- Entrez Gene: 81740 Rat
- Omim: 600299 Human
- SwissProt: Q15154 Human
- SwissProt: Q9R0L6 Mouse
- Unigene: 491148 Human
- Unigene: 117896 Mouse
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Alternative names
- hPCM-1 antibody
- PCM-1 antibody
- pcm1 antibody
see all
Images
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Immunohistochemical analysis of Human testis tissue labeling PCM1 with ab154142 at 1/200 dilution.
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Immunohistochemical analysis of Human cerebral cortex tissue labeling PCM1 with ab154142 at 1/200 dilution.
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Immunohistochemical analysis of Human fallopian tube tissue labeling PCM1 with ab154142 at 1/200 dilution.
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Immunohistochemical analysis of Human dtriated muscle tissue labeling PCM1 with ab154142 at 1/200 dilution.
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Anti-PCM1 antibody [CL0206] (ab154142) at 1/1000 dilution + Human cell line U-251
Predicted band size: 229 kDa -
Anti-PCM1 antibody [CL0206] (ab154142) at 1/1000 dilution + RT4 cell lysate
Predicted band size: 229 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154142 has been referenced in 3 publications.
- Partisani M et al. EFA6A, an exchange factor for Arf6, regulates early steps in ciliogenesis. J Cell Sci 134:N/A (2021). PubMed: 33483367
- Anderson R et al. Length-independent telomere damage drives post-mitotic cardiomyocyte senescence. EMBO J 38:N/A (2019). PubMed: 30737259
- Chavali PL et al. A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer. Nat Commun 7:11005 (2016). PubMed: 26987684