Anti-PCMT1 antibody (ab192790)
Key features and details
- Rabbit polyclonal to PCMT1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCMT1 antibody
See all PCMT1 primary antibodies -
Description
Rabbit polyclonal to PCMT1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human PCMT1 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P22061 -
Positive control
- 293T cell lysate; Human tonsil tissue, A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89% Tris glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
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IHC-P |
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
IHC-P
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Function: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Similarity: Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 5110 Human
- Omim: 176851 Human
- SwissProt: P22061 Human
- Unigene: 279257 Human
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Alternative names
- L isoaspartyl protein carboxyl methyltransferase antibody
- L isoaspartyl/D aspartyl methyltransferase antibody
- L-isoaspartyl protein carboxyl methyltransferase antibody
see all
Images
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Immunofluorescent analysis of methanol fixed A431 cells labeling PCMT1 with ab192790 at 1/200 (green). The cells were costained with Hoechst 33342.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling PCMT1 with an192790 at 7.5 µg/m.
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Anti-PCMT1 antibody (ab192790) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 25 kDa12% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab192790 has not yet been referenced specifically in any publications.