Key features and details
- Rabbit polyclonal to PCMT1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PCMT1 antibody
See all PCMT1 primary antibodies
DescriptionRabbit polyclonal to PCMT1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human PCMT1 aa 10-227. The exact sequence is proprietary.
HSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSP QSIGFQ ATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFAR MVGCTGKVIGIDHI KELVDDSVNNVRKDDPTLLSSGRVQLVVGDGRMG YAEEAPYDAIHVGAAAPVVPQA LIDQLKPGGRLILPVGPAGGNQMLEQ YDKLQDGSIKMKPLMGVIYVPLTDKEKQWS RWK
Database link: P22061
- 293T cell lysate; Human tonsil tissue, A431 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89% Tris glycine, 10% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab192790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 25 kDa.|
|IHC-P||Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceFunction: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Similarity: Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.
- L isoaspartyl protein carboxyl methyltransferase antibody
- L isoaspartyl/D aspartyl methyltransferase antibody
- L-isoaspartyl protein carboxyl methyltransferase antibody
Immunofluorescent analysis of methanol fixed A431 cells labeling PCMT1 with ab192790 at 1/200 (green). The cells were costained with Hoechst 33342.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling PCMT1 with an192790 at 7.5 µg/m.
Anti-PCMT1 antibody (ab192790) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 25 kDa
12% SDS PAGE
ab192790 has not yet been referenced specifically in any publications.