Overview

  • Product name
  • Description
    Rabbit polyclonal to PCMT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-197 of Human PCMT1 (NP_005380)

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates IHC-P: U87 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 1.21% Tris, 0.75% Glycine
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/500.

Target

  • Relevance
    Function: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Similarity: Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • L isoaspartyl protein carboxyl methyltransferase antibody
    • L isoaspartyl/D aspartyl methyltransferase antibody
    • L-isoaspartyl protein carboxyl methyltransferase antibody
    • PCMT 1 antibody
    • Pcmt1 antibody
    • PIMT antibody
    • PIMT_HUMAN antibody
    • Protein beta aspartate methyltransferase antibody
    • Protein L isoaspartate (D aspartate) O methyltransferase antibody
    • Protein L isoaspartyl/D aspartyl methyltransferase antibody
    • Protein L-isoaspartyl/D-aspartyl methyltransferase antibody
    • Protein-beta-aspartate methyltransferase antibody
    • Protein-L-isoaspartate(D-aspartate) O-methyltransferase antibody
    see all

Images

  • Anti-PCMT1 antibody (ab97446) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 25 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of PCMT1 in paraffin embedded U87 xenograft, using ab97446 at a 1/500 dilution.

References

This product has been referenced in:
  • Yamashita M  et al. Deficiency of protein-L-isoaspartate (D-aspartate) O-methyl-transferase expression under endoplasmic reticulum stress promotes epithelial mesenchymal transition in lung adenocarcinoma. Oncotarget 9:13287-13300 (2018). Read more (PubMed: 29568357) »
  • Saito H  et al. Chaperone protein L-isoaspartate (D-aspartyl) O-methyltransferase as a novel predictor of poor prognosis in lung adenocarcinoma. Hum Pathol 50:1-10 (2016). Read more (PubMed: 26997432) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your email. I am very sorry it has taken some time to reply, my Spanish colleague Ariana is currently out of the office. The additional blocking step with 0.3% solution of H2O2 in methanol is suitable for HRP detection methods. I would...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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