Overview

  • Product name

    Anti-PCMTD1 antibody
  • Description

    Rabbit polyclonal to PCMTD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: FVGNQLIPQP LDSEEDEKME EDNKEEEEKD HNEAMKPEEP PQNLLREKIM KLPLPE, corresponding to internal sequence amino acids 290-345 of Human PCMTD1.

  • Positive control

    • Human duodenum tissue, RT-4 cell line lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities

    Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • PCMD1_HUMAN antibody
    • PCMTD1 antibody
    • Protein L isoaspartate (D aspartate) O methyltransferase domain containing 1 antibody
    • Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 antibody

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus. Recommended concentration of ab121858 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121858, at 1/600 dilution, staining PCMTD1 in glandular cells of paraffin-embedded Human duodenum tissue by Immunohistochemistry.
  • All lanes : Anti-PCMTD1 antibody (ab121858) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique.

References

This product has been referenced in:

  • Zhang J  et al. Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun 10:1858 (2019). Read more (PubMed: 31015415) »
See 1 Publication for this product

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