Key features and details
- Mouse monoclonal [5E6/2] to PCNA
- Suitable for: WB, IP, ELISA
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Product nameAnti-PCNA antibody [5E6/2]
See all PCNA primary antibodies
DescriptionMouse monoclonal [5E6/2] to PCNA
Tested applicationsSuitable for: WB, IP, ELISAmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Purified recombinant full length his tagged PCNA from Saccharomyces cerevisiae, produced in Escherichia coli.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab70472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.|
|IP||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Sequence similaritiesBelongs to the PCNA family.
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
- Information by UniProt
- SwissProt: P15873 Saccharomyces cerevisiae
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ab70472 has been referenced in 10 publications.
- Paul Solomon Devakumar LJ et al. Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex. Nucleic Acids Res 47:6826-6841 (2019). PubMed: 31114918
- Gali VK et al. Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation. PLoS Genet 14:e1007783 (2018). PubMed: 30418970
- Shemesh K et al. A structure-function analysis of the yeast Elg1 protein reveals the importance of PCNA unloading in genome stability maintenance. Nucleic Acids Res 45:3189-3203 (2017). WB . PubMed: 28108661
- Johnson C et al. PCNA Retention on DNA into G2/M Phase Causes Genome Instability in Cells Lacking Elg1. Cell Rep 16:684-95 (2016). PubMed: 27373149
- Schalbetter SA et al. Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability. Proc Natl Acad Sci U S A 112:E4565-70 (2015). PubMed: 26240319
- Kubota T et al. Replication-Coupled PCNA Unloading by the Elg1 Complex Occurs Genome-wide and Requires Okazaki Fragment Ligation. Cell Rep 12:774-87 (2015). PubMed: 26212319
- Urulangodi M et al. Local regulation of the Srs2 helicase by the SUMO-like domain protein Esc2 promotes recombination at sites of stalled replication. Genes Dev 29:2067-80 (2015). PubMed: 26443850
- Kubota T et al. The Elg1 replication factor C-like complex functions in PCNA unloading during DNA replication. Mol Cell 50:273-80 (2013). PubMed: 23499004
- Mayfield JA et al. Surrogate genetics and metabolic profiling for characterization of human disease alleles. Genetics 190:1309-23 (2012). PubMed: 22267502
- Kubota T et al. Quantitative Proteomic Analysis of Chromatin Reveals that Ctf18 Acts in the DNA Replication Checkpoint. Mol Cell Proteomics 10:M110.005561 (2011). WB . PubMed: 21505101