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Anti-PCNA antibody [5E6/2] (ab70472)

  • Datasheet
Reviews (1)Q&A (3)References (14)

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Key features and details

  • Mouse monoclonal [5E6/2] to PCNA
  • Reacts with: Saccharomyces cerevisiae
  • Isotype: IgG1

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Protein
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Recombinant Human PCNA protein (ab172197)
Secondary
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Overview

  • Product name

    Anti-PCNA antibody [5E6/2]
    See all PCNA primary antibodies
  • Description

    Mouse monoclonal [5E6/2] to PCNA
  • Host species

    Mouse
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein corresponding to Saccharomyces cerevisiae PCNA. Purified recombinant full length his tagged PCNA from Saccharomyces cerevisiae, produced in Escherichia coli.
    Database link: P15873

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    5E6/2
  • Myeloma

    Sp2
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Markers
    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Binding
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Isotype/Loading Controls
    • Loading Controls
    • PCNA

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human PCNA protein (ab172197)

Target

  • Function

    This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • Sequence similarities

    Belongs to the PCNA family.
  • Post-translational
    modifications

    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • Cellular localization

    Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Target information above from: UniProt accession P12004 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 852385 Saccharomyces cerevisiae
    • SwissProt: P15873 Saccharomyces cerevisiae
    • Alternative names

      • ATLD2 antibody
      • cb16 antibody
      • Cyclin antibody
      • DNA polymerase delta auxiliary protein antibody
      • etID36690.10 antibody
      • fa28e03 antibody
      • fb36g03 antibody
      • HGCN8729 antibody
      • MGC8367 antibody
      • Mutagen-sensitive 209 protein antibody
      • OTTHUMP00000030189 antibody
      • OTTHUMP00000030190 antibody
      • PCNA antibody
      • Pcna/cyclin antibody
      • PCNA_HUMAN antibody
      • PCNAR antibody
      • Polymerase delta accessory protein antibody
      • Proliferating cell nuclear antigen antibody
      • wu:fa28e03 antibody
      • wu:fb36g03 antibody
      see all

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (14)

    Publishing research using ab70472? Please let us know so that we can cite the reference in this datasheet.

    ab70472 has been referenced in 14 publications.

    • Reyes GX  et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair. Curr Biol 31:1268-1276.e6 (2021). PubMed: 33417883
    • Guilliam TA & Yeeles JTP Reconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart. Nat Struct Mol Biol 27:450-460 (2020). PubMed: 32341533
    • Kulkarni DS  et al. PCNA activates the MutL? endonuclease to promote meiotic crossing over. Nature 586:623-627 (2020). PubMed: 32814343
    • Paul Solomon Devakumar LJ  et al. Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex. Nucleic Acids Res 47:6826-6841 (2019). PubMed: 31114918
    • Huang Y  et al. miR-101 regulates the cell proliferation and apoptosis in diffuse large B-cell lymphoma by targeting MEK1 via regulation of the ERK/MAPK signaling pathway. Oncol Rep 41:377-386 (2019). PubMed: 30365139
    View all Publications for this product

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody [5E6/2]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100
    Read More

    DR. Kirk Mcmanus

    Verified customer

    Submitted Oct 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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