Anti-PCNA antibody [5E6/2] (ab70472)
Key features and details
- Mouse monoclonal [5E6/2] to PCNA
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-PCNA antibody [5E6/2]
See all PCNA primary antibodies -
Description
Mouse monoclonal [5E6/2] to PCNA -
Host species
Mouse -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein corresponding to Saccharomyces cerevisiae PCNA. Purified recombinant full length his tagged PCNA from Saccharomyces cerevisiae, produced in Escherichia coli.
Database link: P15873 -
General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5E6/2 -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Target
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Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
Sequence similarities
Belongs to the PCNA family. -
Post-translational
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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Database links
- Entrez Gene: 852385 Saccharomyces cerevisiae
- SwissProt: P15873 Saccharomyces cerevisiae
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Alternative names
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (14)
ab70472 has been referenced in 14 publications.
- Reyes GX et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair. Curr Biol 31:1268-1276.e6 (2021). PubMed: 33417883
- Guilliam TA & Yeeles JTP Reconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart. Nat Struct Mol Biol 27:450-460 (2020). PubMed: 32341533
- Kulkarni DS et al. PCNA activates the MutL? endonuclease to promote meiotic crossing over. Nature 586:623-627 (2020). PubMed: 32814343
- Paul Solomon Devakumar LJ et al. Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex. Nucleic Acids Res 47:6826-6841 (2019). PubMed: 31114918
- Huang Y et al. miR-101 regulates the cell proliferation and apoptosis in diffuse large B-cell lymphoma by targeting MEK1 via regulation of the ERK/MAPK signaling pathway. Oncol Rep 41:377-386 (2019). PubMed: 30365139
