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Anti-PCNA antibody (ab18197)

  • Datasheet
  • SDS
Reviews (47)Q&A (1)References (203)

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Western blot - Anti-PCNA antibody (ab18197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
  • Flow Cytometry - Anti-PCNA antibody (ab18197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
  • Western blot - Anti-PCNA antibody (ab18197)

Key features and details

  • Rabbit polyclonal to PCNA
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human, Common marmoset
  • Isotype: IgG

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Overview

  • Product name

    Anti-PCNA antibody
    See all PCNA primary antibodies
  • Description

    Rabbit polyclonal to PCNA
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Mouse
    Common marmoset
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human PCNA aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P12004
    (Peptide available as ab18602, ab2427)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Markers
    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Binding
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Isotype/Loading Controls
    • Loading Controls
    • PCNA

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PCNA protein (ab172197)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • KN-62, CaM kinase II inhibitor. P2X7 antagonist. (ab120421)
    • Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Mouse
Human
IHC-P
Mouse
Common marmoset
WB
Human
All applications
Sheep
Goat
Cow
Dog
Xenopus laevis
Monkey
Zebrafish
Application Abreviews Notes
WB (10)
Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ICC/IF (13)
Use a concentration of 1 µg/ml.
Flow Cyt
Use 0.05µg for 106 cells.
IHC-P (20)
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use 0.05µg for 106 cells.
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • Sequence similarities

    Belongs to the PCNA family.
  • Post-translational
    modifications

    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • Cellular localization

    Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Target information above from: UniProt accession P12004 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 515499 Cow
    • Entrez Gene: 477166 Dog
    • Entrez Gene: 5111 Human
    • Entrez Gene: 18538 Mouse
    • Entrez Gene: 25737 Rat
    • Entrez Gene: 30678 Zebrafish
    • Omim: 176740 Human
    • SwissProt: Q3ZBW4 Cow
    • SwissProt: P12004 Human
    • SwissProt: P17918 Mouse
    • SwissProt: P04961 Rat
    • SwissProt: P18248 Xenopus laevis
    • SwissProt: Q9PTP1 Zebrafish
    • Unigene: 147433 Human
    • Unigene: 728886 Human
    • Unigene: 7141 Mouse
    • Unigene: 223 Rat
    • Unigene: 34965 Xenopus laevis
    • Unigene: 35605 Zebrafish
    see all
  • Alternative names

    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

Images

  • Western blot - Anti-PCNA antibody (ab18197)
    Western blot - Anti-PCNA antibody (ab18197)
    All lanes : Anti-PCNA antibody (ab18197) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Human PCNA peptide (ab18602) at 1 µg/ml
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Human PCNA peptide (ab18602) at 1 µg/ml
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate with Human PCNA peptide (ab18602) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa
    Additional bands at: 48 kDa, 52 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)Image courtesy of Carl Hobbs, Kings College London, U.K.

    ab18197 staining PCNA in tissue sections of the goat spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/4000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)This image is courtesy of an Abreview submitted by Dr Kirk McManus
    ab18197, at a 1/5000 dilution, staining PCNA in assynchronous HeLa cells. Cells were counter-stained with DAPI (red). For more information please refer to Abreview.

    See Abreview

  • Flow Cytometry - Anti-PCNA antibody (ab18197)
    Flow Cytometry - Anti-PCNA antibody (ab18197)

    Overlay histogram showing HeLa cells stained with ab18197 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18197, 0.05μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (ab171870, 0.05μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)Image courtesy of Carl Hobbs, Kings College London, U.K.

    ab18197 staining PCNA in tissue sections of the marmoset spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/6000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)Image courtesy of Carl Hobbs, King College London, U.K.

    ab18197 staining PCNA in tissue sections of the cow spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/4000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)Image courtesy of Carl Hobbs, King College London, U.K.

    ab18197 staining PCNA in tissue sections of the sheep spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/6000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)Image courtesy of Carl Hobbs, Kings College London, U.K.

    ab18197 staining PCNA in tissue sections of the rat brain by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/10000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)Image courtesy of Carl Hobbs, Kings College London, U.K.

    ab18197 staining PCNA in tissue sections of the mouse brain by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/6000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab18197)Image Courtesy of Carl Hobbs, Kings College London, U.K.

    ab18197 staining PCNA in monkey COS cell pellet by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/4000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)

    ICC/IF image of ab18197 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18197, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)Image courtesy of Hank Farr by Abreview.
    ab18197 staining PCNA in Zebrafish gastrula embryos by Immunocytochemistry/ Immunofluorescence (wholemount).
    Zebrafish embryos were fixed overnight at 4°C when they had reached 60% epiboly. Cells were fixed in formaldehyde, permeabilized using Proteinase K, blocked with 2% goat serum for 2 hours at 20°C and then incubated with ab18197 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution. Cells were post-fixed in PFA for 20 minutes at room temperature after extensive washing of the secondary antibody.

    The left panel shows DAPI stained nuclei, the center panel is PCNA staining, and the right panel is the merged image.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)

    ICC/IF image of ab18197 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18197, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    Panel A does not show the Alexa Fluor® 488 channel, Panel B shows the specfic nuclear staining by ab18197.
  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)This image is courtesy of an abreview submitted by Dr Catherine Green
    ab18197, at a 1/2000 dilution staining PCNA in MRC5 Sv40 transformed fibroblasts. Cells were counterstained with DAPI (blue). For more information please refer to Abreview.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
    Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab18197)
    ab18197 staining PCNA in SK-N-SH cells treated with KN-62 (ab120421), by ICC/IF. Increase in PCNA nuclear expression correlates with increased concentration of KN-62, as described in literature.
    The cells were incubated at 37øC for 24h in media containing different concentrations of ab120421 (KN-62) in DMSO, fixed with 100% methanol for 5 minutes at -20øC and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18197 (1 æg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Western blot - Anti-PCNA antibody (ab18197)
    Western blot - Anti-PCNA antibody (ab18197)
    Anti-PCNA antibody (ab18197) at 1 µg/ml + Recombinant Human PCNA protein (ab85651) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa


    Exposure time: 10 seconds

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (203)

Publishing research using ab18197? Please let us know so that we can cite the reference in this datasheet.

ab18197 has been referenced in 203 publications.

  • Tian Y  et al. Silencing of RHEB inhibits cell proliferation and promotes apoptosis in colorectal cancer cells via inhibition of the mTOR signaling pathway. J Cell Physiol 235:442-453 (2020). PubMed: 31332784
  • Huang L  et al. Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/ß-catenin axis. J Exp Clin Cancer Res 39:63 (2020). PubMed: 32293515
  • Fan Z  et al. MicroRNA-24 attenuates diabetic vascular remodeling by suppressing the NLRP3/caspase-1/IL-1ß signaling pathway. Int J Mol Med 45:1534-1542 (2020). PubMed: 32323758
  • Liu J  et al. Knockdown of circAPLP2 Inhibits Progression of Colorectal Cancer by Regulating miR-485-5p/FOXK1 Axis. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32343603
  • Zeng Z  et al. IL-10 regulates the malignancy of hemangioma-derived endothelial cells via regulation of PCNA. Arch Biochem Biophys 688:108404 (2020). PubMed: 32416101
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 48 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (epithelial cells)
Specification
epithelial cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Dr. Tina Yuan

Verified customer

Submitted Sep 17 2019

Immunohistochemistry (Frozen sections) abreview for Anti-PCNA antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (bone marrow cells)
Permeabilization
No
Specification
bone marrow cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 17 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Bone marrow)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9.0
Permeabilization
No
Specification
Bone marrow
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 16 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
Permeabilization
Yes - 0.05% triton-x100
Specification
Bone
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 28 2019

Immunocytochemistry abreview for Anti-PCNA antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cell (bone marrow)
Permeabilization
Yes - 0.05% triton-x100
Specification
bone marrow
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Dr. Hanson Lee

Verified customer

Submitted Aug 27 2019

Immunohistochemistry (Frozen sections) abreview for Anti-PCNA antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (alveolar bone)
Permeabilization
No
Specification
alveolar bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
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Dr. Tina Yuan

Verified customer

Submitted Jun 16 2019

Western blot abreview for Anti-PCNA antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary cardiac fibrobalsts)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
primary cardiac fibrobalsts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Mrs. talya razin

Verified customer

Submitted Jun 11 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus tropicalis Cell (Tadpole brain whole-mount)
Permeabilization
Yes - 0.1% TritonX100
Specification
Tadpole brain whole-mount
Blocking step
CAS-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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Helen Willsey

Verified customer

Submitted May 01 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic mouse brain tissue)
Permeabilization
Yes - 0.3% Triton X 100
Specification
Embryonic mouse brain tissue
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Oct 09 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Gerbil Tissue sections (brain)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Formaldehyde
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durgesh dwivedi

Verified customer

Submitted Aug 03 2018

1-10 of 48 Abreviews or Q&A

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