Immunostaining was performed directly in corneal tissue. 0,5% SDS at RT for 5 min may improve the staining quality after tissue fixation with 0,5% PFA
I have used the epithelium itself in organ culture (in situ condition), but not the in vitro cultured epithelial cells in vitro. Because of this special tissue condition, I used always whole gaot serum to prevent the non specifical satining due to the sencondary antibody (goat anti rabbit) Thank you for your attention and your standard protocol.
We appreciate the provided information. In standart protocols for IHC blocking
Dr. Zhiguo He
Submitted Nov 08 2012
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