Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody - Proliferation Marker

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (corneal epithelium)
Specification
corneal epithelium
Fixative
Formaldehyde
Permeabilization
Yes - 1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C

Other product details

Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 2% BSA 2% goat serum in PBS

Secondary antibody

Name
Non-Abcam antibody was used: alexa fluor 555 goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/500

Additional data

PubMed ID
22219645: View
Additional Notes
Immunostaining was performed directly in corneal tissue. 0,5% SDS at RT for 5 min may improve the staining quality after tissue fixation with 0,5% PFA

I have used the epithelium itself in organ culture (in situ condition), but not the in vitro cultured epithelial cells in vitro. Because of this special tissue condition, I used always whole gaot serum to prevent the non specifical satining due to the sencondary antibody (goat anti rabbit) Thank you for your attention and your standard protocol.
Abcam response
We appreciate the provided information. In standart protocols for IHC blocking

Dr. Zhiguo He

Verified customer

Submitted Nov 08 2012

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