Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody - Proliferation Marker

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (corneal endothelial cells in vitro)
Specification
corneal endothelial cells in vitro
Fixative
Formaldehyde
Permeabilization
Yes - 1% triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C

Other product details

Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 2% BSA, 2% goat serum

Secondary antibody

Name
Non-Abcam antibody was used: Alexa fluor 555 goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

PubMed ID
22219645: View
Additional Notes
4% PFA fixation

Dr. Zhiguo He

Verified customer

Submitted Nov 06 2012

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