Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody - Proliferation Marker

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/6000

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
Submitted image of the svz of the Lateral ventricle shows positivity confined, mostly, to nuclei of progenitor olfactory neurones. There is a slight background in the neuropil that I cannot get rid of, without losing specific signal.

Mr. Carl Hobbs

Verified customer

Submitted Nov 01 2013

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