Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Cow Tissue sections (Spleen)
Specification
Spleen
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/4000

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
There is a slight extracellular positivity: this may well be due to the fact that this tissue seems to be sub-optimally preserved. This does not, however, mask specific positivity.

Mr. Carl Hobbs

Verified customer

Submitted Dec 13 2013

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