Western blot abreview for Anti-PCNA antibody

Below Average
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS Tween 0,5% Lait 5% NaN3
Dilution
1/200

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL Clarity
Exposure
4 second(s)
Bands
Specific: 0 kDa Non-specific: 54 kDa

Additional data

Additional Notes
Lane 1: ladder Lane 2: Human intestinal tissue lysate
Abcam response
Our Scientific Support team has contacted this researcher regarding this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

Mr. Julien Chevalier

Verified customer

Submitted Jul 08 2016

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