Western blot abreview for Anti-PCNA antibody

Average
Abreviews
Application
Western blot
Sample
Pig Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
25 µg
Specification
Intestinal tissue
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS Tween 0,5% Lait 5% NaN3
Dilution
1/200

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL Clarity
Exposure
4 second(s)
Bands
Specific: 33 kDa Non-specific: 80 kDa

Additional data

Additional Notes
Lane 1: ladder Lane 2: mouse intestinal tissue lysate Lane 3: pig intestinal tissue lysate

Mr. Julien Chevalier

Verified customer

Submitted Jul 08 2016

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