Western blot abreview for Anti-PCNA antibody

Excellent
Abreviews
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12%)
Loading amount
15 µg
Specification
MDCK
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Detection

Detection method
Clarity western ECL substrate
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 32 kDa Non-specific: 48 kDa

Additional data

Additional Notes
ABTRIAL-EVTN3

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Submitted Nov 25 2016

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