Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PCNA antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Diluent: TBS
Dilution
1/1000

Secondary antibody

Dilution
1/600
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 647

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Submitted Jan 31 2018

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