Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic mouse brain tissue)
Permeabilization
Yes - 0.3% Triton X 100
Specification
Embryonic mouse brain tissue
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Other product details

Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% NGS+0.3% Tritonx 100 in PBS
Dilution
1/800

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
the cryosections were antigen retreived in 10mM sodium citrate buffer (pH 6) by boiling the slides in a microwave for 2 min.

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Submitted Oct 09 2018

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