Key features and details
- Rabbit polyclonal to PCNA
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Product nameAnti-PCNA antibody
See all PCNA primary antibodies
DescriptionRabbit polyclonal to PCNA
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant full length protein corresponding to Saccharomyces cerevisiae PCNA aa 1-258.
MLEAKFEEASLFKRIIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSL EIGVEAFQEY RCDHPVTLGMDLTSLSKILRCGNNTDTLTLIADNTPDS IILLFEDTKKDRIAEYSLKLMD IDADFLKIEELQYDSTLSLPSSEFSK IVRDLSQLSDSINIMITKETIKFVADGDIGSGSV IIKPFVDMEHPETS IKLEMDQPVDLTFGAKYLLDIIKGSSLSDRVGIRLSSEAPALFQFD LK SGFLQFFLAPKFNDEE
Database link: P15873
- Crude extract of S cerevisiae strain BY4741.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab221196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 29 kDa.|
FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Sequence similaritiesBelongs to the PCNA family.
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
- Information by UniProt
- SwissProt: P15873 Saccharomyces cerevisiae
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
ab221196 has been referenced in 1 publication.
- Janke R et al. Pivotal roles of PCNA loading and unloading in heterochromatin function. Proc Natl Acad Sci U S A 115:E2030-E2039 (2018). PubMed: 29440488