Product nameAnti-PCNA antibody [BLR075G]
See all PCNA primary antibodies
DescriptionRabbit monoclonal [BLR075G] to PCNA
Tested applicationsSuitable for: ICC, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PCNA aa 225-261. The exact sequence is proprietary. NP_002583.1
Database link: P12004
- WB: A549, Jurkat, HEK-293T, RKO, K562, MCF7, HeLa and U-2 OS whole cell lysate. IHC-P: Human prostate carcinoma and ovarian carcinoma tissue. ICC: OVCAR-3 cells.
This product is sold under License from Bethyl Laboratories, Inc
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab265609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/100 - 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 29 kDa.|
FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Sequence similaritiesBelongs to the PCNA family.
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
- Information by UniProt
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
All lanes : Anti-PCNA antibody [BLR075G] (ab265609) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : RKO whole cell lysate
Lane 5 : K562 whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : U-2 OS whole cell lysate
Lane 9 : TCMK whole cell lysate
Lane 10 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 29 kDa
Exposure time: 30 seconds
Cells prepared using NETN lysis buffer.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265609 has not yet been referenced specifically in any publications.