Immunocytochemistry/ Immunofluorescence abreview for Anti-PCNA antibody - Nuclear Loading Control

Immunocytochemistry/ Immunofluorescence

Human Cell (Ovarian Cancer cell lines OVCAR3 and SKOV2)

Yes - Triton X100

Ovarian Cancer cell lines OVCAR3 and SKOV2

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 23°C

Paraformaldehyde

1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 1.5% BSA

1/500

1/500

Non-Abcam antibody was used: goat anti chicken highly cross adsorbed, alexa flu
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

The antibody was used in the 4i protocol as described in Science. 2018 Aug 3361(6401) - Multiplexed protein maps link subcellular organization to cellular states - G. Gut. Tested in 2 dilutions 1/500 and 1/5000 - the ideal dilution is somewhere around 1/2000 for IF The image shows an overlay of DAPI in blue and PCNA in red