Product nameAnti-PCNA antibody [PC10]
See all PCNA primary antibodies
DescriptionMouse monoclonal [PC10] to PCNA
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Rat PCNA.
Database link: P12004
- IHC-P: Human colon carcinoma tissue and mouse colon carcinoma tissue. ICC/IF: H226 cells. WB: TCMK-1, NIH/3T3, HeLa and jurkat cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab265585 in the following tested applications.
|WB||1/1000. Predicted molecular weight: 29 kDa.|
|IP||Use at an assay dependent concentration.
20 µl/1 mg lysate
|IHC-P||1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
|ICC/IF||1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections
FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Sequence similaritiesBelongs to the PCNA family.
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
- Information by UniProt
- ATLD2 antibody
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB
PCNA was immunoprecipitated from 1 mg TCMK-1 cell lysate using ab265585 at 20 µl/mg lysate. Western blot was performed on the immunoprecipitate using ab265585 at 1/1000 dilution.
Lane 1: ab265585 IP in TCMK-1 whole cell lysate.
Lane 2: Control IgG.
All lanes : Anti-PCNA antibody [PC10] (ab265585) at 1/1000 dilution
Lane 1 : TCMK-1 whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP conjugated goat anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 1 second
Formalin-fixed, paraffin-embedded H226 cells stained for PCNA using ab265585 at 1/100 dilution in ICC/IF analysis. Substrate: DAB.
Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for PCNA using ab265585 at 1/100 dilution in immunohistochemical analysis. Substrate: DAB
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265585 has not yet been referenced specifically in any publications.