Key features and details
- Rabbit polyclonal to PCNP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PCNP antibody
See all PCNP primary antibodies
DescriptionRabbit polyclonal to PCNP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
- HeLa, 293T and Jurkat whole cell lysates prepared using NETN lysis buffer.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab200551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000. Predicted molecular weight: 19 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay be involved in cell cycle regulation.
modificationsUbiquitinated; mediated by UHRF2 and leading to its subsequent proteasomal degradation.
- Information by UniProt
- Ab2 416 antibody
- AI647035 antibody
- PCNP antibody
Immunoprecipitation of 293T whole cell lysate (0.5 or 1.0 mg per IP reaction) using ab200551 at 6 µg per reaction. For WB, immunoprecipitated PCNP (20% of IP loaded), was used at 0.04 µg/mL (lane 1) and control IgG (lane 2).
All lanes : Anti-PCNP antibody (ab200551) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 19 kDa
Exposure time: 30 seconds
ab200551 has not yet been referenced specifically in any publications.