Overview

  • Product name

  • Description

    Rabbit polyclonal to PCNP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PCNP aa 1-50. The exact sequence is proprietary. NP_065090.1
    Sequence:

    MADGKAGDEK PEKSQRAGAA GGPEEEAEKP VKTKTVSSSN GGESSSRSAE


    Database link: Q8WW12

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates prepared using NETN lysis buffer.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000. Predicted molecular weight: 19 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May be involved in cell cycle regulation.
  • Post-translational
    modifications

    Ubiquitinated; mediated by UHRF2 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ab2 416 antibody
    • AI647035 antibody
    • PCNP antibody
    • PCNP_HUMAN antibody
    • PEST containing nuclear protein antibody
    • PEST proteolytic signal containing nuclear protein antibody
    • PEST proteolytic signal-containing nuclear protein antibody
    • PEST-containing nuclear protein antibody
    see all

Images

  • Immunoprecipitation of 293T whole cell lysate (0.5 or 1.0 mg per IP reaction) using ab200551 at 6 µg per reaction. For WB, immunoprecipitated PCNP (20% of IP loaded), was used at 0.04 µg/mL (lane 1) and control IgG (lane 2).

  • All lanes : Anti-PCNP antibody (ab200551) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 19 kDa


    Exposure time: 30 seconds

References

ab200551 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab200551.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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