Anti-PCOLCE antibody (ab220040)
Key features and details
- Rabbit polyclonal to PCOLCE
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCOLCE antibody
See all PCOLCE primary antibodies -
Description
Rabbit polyclonal to PCOLCE -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PCOLCE aa 250-400.
Database link: Q15113 -
Positive control
- ICC/IF: U-2 OS cells. WB: U-251 MG cell lysate, human plasma and tonsil tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 48 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 48 kDa. |
Target
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Function
Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.
C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity. -
Sequence similarities
Contains 2 CUB domains.
Contains 1 NTR domain. -
Post-translational
modificationsC-terminally processed at multiple positions. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5118 Human
- Omim: 600270 Human
- SwissProt: Q15113 Human
- Unigene: 202097 Human
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Alternative names
- PCOC1_HUMAN antibody
- Pcolce antibody
- PCOLE 1 antibody
see all
Images
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Immunofluorescent analysis of formalin fixed, triton X-100 permeabilized U-2 OS cells labeling PCOLCE using ab220040 at 4 μg/ml (green).
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All lanes : Anti-PCOLCE antibody (ab220040) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 48 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab220040 has not yet been referenced specifically in any publications.