Anti-PCOLCE antibody (ab220040)

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Overview

  • Product name

    Anti-PCOLCE antibody
    See all PCOLCE primary antibodies

  • Description

    Rabbit polyclonal to PCOLCE

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human PCOLCE aa 298-373.
    Sequence:

    PKSQPPEKTEESPSAPDAPTCPKQCRRTGTLQSNFCASSLVVTATVKSMV REPGEGLAVTVSLIGAYKTGGLDLPS


    Database link: Q15113

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF: U-2 OS cells. WB: U-251 MG cell lysate, human plasma and tonsil tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab220040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.
WB 1/100 - 1/250. Predicted molecular weight: 48 kDa.

Target

  • Function

    Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.
    C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity.

  • Sequence similarities

    Contains 2 CUB domains.
    Contains 1 NTR domain.

  • Post-translational
    modifications

    C-terminally processed at multiple positions.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • PCOC1_HUMAN antibody
      • Pcolce antibody
      • PCOLE 1 antibody
      • PCOLE1 antibody
      • PCPE antibody
      • PCPE-1 antibody
      • PCPE1 antibody
      • Procollagen C endopeptidase enhancer antibody
      • Procollagen C-endopeptidase enhancer 1 antibody
      • Procollagen C-endopeptidase enhancer antibody
      • Procollagen C-proteinase enhancer 1 antibody
      • Procollagen COOH-terminal proteinase enhancer 1 antibody
      • procollagen, type 1, COOH-terminal proteinase enhancer antibody
      • Type 1 procollagen C-proteinase enhancer protein antibody
      • Type I procollagen COOH-terminal proteinase enhancer antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PCOLCE antibody (ab220040)
      Immunocytochemistry/ Immunofluorescence - Anti-PCOLCE antibody (ab220040)

      Immunofluorescent analysis of formalin fixed, triton X-100 permeabilized U-2 OS cells labeling PCOLCE using ab220040 at 4 μg/ml (green).

    • Western blot - Anti-PCOLCE antibody (ab220040)
      Western blot - Anti-PCOLCE antibody (ab220040)
      All lanes : Anti-PCOLCE antibody (ab220040) at 1/100 dilution

      Lane 1 : RT-4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human plasma lysate
      Lane 4 : Human liver tissue lysate
      Lane 5 : Human tonsil tissue lysate

      Predicted band size: 48 kDa

    References

    ab220040 has not yet been referenced specifically in any publications.

    Publishing research using ab220040? Please let us know so that we can cite the reference in this datasheet.

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