Overview

  • Product name
  • Description
    Rabbit polyclonal to PCOLE2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 201-230 of Human PCOLE2 (NP_037495.1).

  • Positive control
    • 293 cell line lysates; mouse heart tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab156224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 46 kDa.

Target

  • Function
    Binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1.
  • Tissue specificity
    Highly expressed in the heart, trabecular meshwork, pituitary gland, bladder, mammary gland, trachea and placenta and weakly expressed in the brain. Expressed in cartilage.
  • Sequence similarities
    Contains 2 CUB domains.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    O-glycosylated; contains sialic acid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2400001O18Rik antibody
    • MGC187469 antibody
    • PCOC2_HUMAN antibody
    • PCOLCE 2 antibody
    • Pcolce2 antibody
    • PCOLE 2 antibody
    • PCPE 2 antibody
    • PCPE-2 antibody
    • PCPE2 antibody
    • Procollagen C endopeptidase enhancer 2 antibody
    • Procollagen C proteinase enhancer 2 antibody
    • Procollagen C-endopeptidase enhancer 2 antibody
    • Procollagen C-proteinase enhancer 2 antibody
    • Procollagen COOH terminal proteinase enhancer 2 antibody
    • Procollagen COOH-terminal proteinase enhancer 2 antibody
    see all

Images

  • Anti-PCOLE2 antibody (ab156224) at 1/100 dilution + 293 cell line lysates at 35 µg

    Predicted band size: 46 kDa

  • Anti-PCOLE2 antibody (ab156224) at 1/100 dilution + mouse heart tissue lysates at 35 µg

    Predicted band size: 46 kDa

References

ab156224 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Sample
Cynomolgus Monkey Serum (serum, PBS diluted)
Specification
serum, PBS diluted
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

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Verified customer

Submitted Jun 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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