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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 0.4 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus.
Expressed in brain, placenta, small intestine, stomach, uterus and weakly in the prostate. Isoform alpha has been observed only in the brain. Isoform gamma is expressed in brain, placenta and uterus. Isoform delta is expressed in brain, kidney, placenta, prostate, small intestine and uterus.
Belongs to the protein-tyrosine phosphatase family. Receptor class 7 subfamily. Contains 1 tyrosine-protein phosphatase domain.
Cell membrane and Cytoplasm > perinuclear region. Locates to the perinuclear areas within the cytoplasm.
Protein tyrosine phosphatase Cr1PTPase precursor antibody
Protein tyrosine phosphatase NC PTPCOM1 antibody
Protein tyrosine phosphatase PCPTP1 antibody
Protein tyrosine phosphatase receptor type R antibody
Protein-tyrosine phosphatase PCPTP1 antibody
PTP SL antibody
Receptor type tyrosine protein phosphatase R antibody
Receptor-type tyrosine-protein phosphatase R antibody
Western blot - Anti-PCPTP1 antibody (ab12153)Phosphatase domain fusion proteins provided by Purely Proteins
All lanes : Anti-PCPTP1 antibody (ab12153) at 0.4 µg/ml
Lane 1 : 0.01µg PTP beta (PTP RB) Lane 2 : 0.01µg PTP epsilon (PTP RE) Lane 3 : 0.01µg LAR (PTP RF) Lane 4 : 0.01µg PTP gamma (PTP RG) Lane 5 : 0.01µg SAP1 (PTP RH) Lane 6 : 0.01µg DEP1 (PTP RJ) Lane 7 : 0.01µg PTP kappa (PTP RK) Lane 8 : 0.01µg PTPIA2 (PTP RN) Lane 9 : 0.01µg GLEPP1 (PTP RO) Lane 10 : 0.01µg PCPTP1 (PTP RR) Lane 11 : 0.01µg CD45 (PTP RC)
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 0.2 µg/ml
Performed under reducing conditions.
Predicted band size: 73 kDa Observed band size: 73 kDa
Recombinant PTP phosphatase domains (0.01µg per lane) were probed with ab12153 at 1/2500 followed by secondary goat anti-rabbit IgG antibody (ab6721 at 1/5000):
Exposure time: 30s
Lane 1: PTP beta (PTP RB) Lane 2: PTP epsilon (PTP RE) Lane 3: LAR (PTP RF) Lane 4: PTP gamma (PTP RG) Lane 5: SAP1 (PTP RH) Lane 6: DEP1 (PTP RJ) Lane 7: PTP kappa (PTP RK) Lane 8: PTPIA2 (PTP RN) Lane 9: GLEPP1 (PTP RO) Lane 10: PCPTP1 (PTP RR) Lane 11: CD45 (PTP RC)
Recombinant PCPTP1 phosphatase domains (0.01µg per lane) were probed with ab12153 at 0.4µg/ml
ab12153 staining PCPTP1 in Human cerebellum. The paraffin embedded tissue was incubated with ab12153 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab12153 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.